MUHWA Ready-Mixed PBS Powder — Your Go-To Buffer for Cell & Molecular Biology
MUHWA ready-mixed PBS (Phosphate Buffered Saline) powder is a pre-formulated, granular buffer designed for convenience and consistency. Each bag dissolves in distilled water to produce 2 liters of 0.01 M PBS at pH 7.2–7.4—no weighing, no pH titration, no hassle. The standardized formulation (NaCl, KCl, Na₂HPO₄, KH₂PO₄) is isotonic and compatible with a broad range of laboratory protocols, from cell culture washing to immunohistochemistry and ELISA. With 10 bags per pack, you get 20 liters of ready-to-use buffer at an economical price point.
Key Features
- Pre-Formulated & Ready to Mix: No separate reagent weighing. Tear open a bag, add to distilled water, stir, and use.
- pH-Stable: Consistently yields pH 7.2–7.4 at 25°C when prepared as directed. No additional pH adjustment needed.
- Isotonic & Biocompatible: 0.01 M PBS is isotonic to mammalian cells; suitable for cell washing, resuspension, and short-term incubation.
- High Purity & Solubility: White granular powder dissolves rapidly to a clear, particle-free solution. Low endotoxin for sensitive applications.
- 10 Bags × 2 L = 20 L Total: Bulk packaging reduces per-liter cost for high-throughput labs and teaching laboratories.
- Versatile Buffer System: Use as washing buffer, diluent for antibodies/proteins/nucleic acids, chromatography equilibration buffer, and more.
PBS Composition (per 2 L bag, 0.01 M)
| Component | Formula | MW (g/mol) | Concentration (mM) |
| Sodium Chloride | NaCl | 58.44 | 137 |
| Potassium Chloride | KCl | 74.55 | 2.7 |
| Sodium Phosphate Dibasic | Na₂HPO₄ | 141.96 | 10 |
| Potassium Phosphate Monobasic | KH₂PO₄ | 136.09 | 1.76 |
Preparation Instructions
- Add 1.6 L of distilled/deionized water to a suitable container.
- Add 1 bag of MUHWA PBS ready-mixed powder.
- Stir until completely dissolved (∼2–5 minutes with magnetic stirring).
- Adjust volume to 2 L with additional distilled water.
- Verify pH (7.2–7.4). No adjustment is typically required.
- Filter-sterilize (0.22 µm) if aseptic buffer is needed for cell culture.
Applications
- Cell culture washing and rinsing (mammalian, insect, and primary cells)
- Immunohistochemistry (IHC) and immunocytochemistry (ICC) wash steps
- ELISA coating and washing buffer
- Protein and antibody dilution buffer
- DNA/RNA resuspension and dilution
- Chromatography column equilibration
- General biochemistry and molecular biology workflows
FAQ
Q: Can this PBS be used for cell culture?
Yes. The formulation is isotonic and biocompatible. For aseptic cell culture, filter-sterilize through a 0.22 µm membrane after preparation.
Q: Do I need to adjust the pH?
No. When prepared as directed, the solution consistently yields pH 7.2–7.4 at room temperature. Verify with a calibrated pH meter for critical applications.
Q: How should I store the prepared solution?
Store prepared 1× PBS at 2–8°C for up to 4 weeks, or at room temperature for up to 2 weeks. Discard if turbidity or microbial growth is observed.
Q: What is the difference between this and 10× PBS concentrate?
This is a 1× ready-mixed powder — just add water. 10× PBS concentrates require dilution and are typically used for large-volume buffer preparation. Our ready-mixed bags eliminate the dilution step.
