MUHWA 0.5mL Thin Wall PCR Tubes — Precision for Every Reaction
The MUHWA 0.5 mL thin-wall PCR tube is a high-quality polypropylene consumable specifically designed for polymerase chain reaction (PCR) applications. Featuring an integrated flat cap and ultra-thin wall construction, these tubes ensure optimal thermal transfer and sample integrity across a wide range of thermal cyclers. Each tube is manufactured under strict quality control to be RNase-free, DNase-free, and non-pyrogenic — meeting the demands of sensitive molecular biology protocols.
Key Features
- Ultra-Thin Wall Construction: Uniform wall thickness ensures rapid and even heat conduction for consistent amplification results across all wells.
- Integrated Flat Cap: Secure, leak-proof seal prevents evaporation and cross-contamination during thermal cycling; ergonomic design for easy one-handed operation with gloved hands.
- High Transparency: Crystal-clear virgin polypropylene allows easy visual monitoring of reaction volumes, sample color, and pellet formation.
- Certified Clean: RNase-free, DNase-free, and non-pyrogenic — tested and certified for sensitive enzymatic reactions and clinical research applications.
- Non-Graduated Interior: Smooth, non-graduated inner surface minimizes sample adhesion and maximizes recovery of precious reagents.
- High-g Centrifugation: Withstands forces up to 10,000 g for pre- and post-PCR spin-down and purification steps.
Technical Specifications
| Material | Virgin Polypropylene (PP) |
| Capacity | 0.5 mL |
| Cap Type | Integrated Flat Cap |
| Wall Type | Thin Wall |
| Sterility | Non-Sterile (RNase/DNase-free, Non-pyrogenic) |
| Max RCF | 10,000 × g |
| Graduation | Non-Graduated |
| Color | Clear / Transparent |
| Package Quantity | 1,000 Tubes per Pack |
| Autoclavable | Yes (121°C, 20 min) |
Applications
- Standard PCR and endpoint PCR
- Quantitative real-time PCR (qPCR)
- Reverse transcription PCR (RT-PCR)
- DNA amplification and genotyping
- Molecular cloning and sequencing preparation
- Clinical diagnostics and research laboratory workflows
FAQ
Q: Are these tubes compatible with all thermal cyclers?
Yes. These 0.5 mL PCR tubes fit standard 0.5 mL thermal cycler blocks (96-well format for 0.5 mL). For 0.2 mL cycler blocks, see our 0.2 mL PCR tube offering.
Q: Are the tubes sterile?
These tubes are supplied non-sterile but are certified RNase-free, DNase-free, and non-pyrogenic. Autoclave at 121°C for 20 minutes if sterility is required for your protocol.
Q: Can these be used for sample storage?
Yes. The secure flat cap and polypropylene construction make them suitable for short-term sample storage from -20°C to room temperature. For long-term cryogenic storage, we recommend dedicated cryovials.
Q: Thin wall vs. standard wall — why does it matter?
Thin-wall tubes offer faster, more uniform thermal transfer — critical for consistent denaturation and annealing across all samples. Standard-wall tubes equilibrate more slowly and may create temperature gradients that compromise reaction uniformity.