MUHWA 4mL Amber HPLC Vials — 13-425 Screw Cap with PTFE/Silicone Septa, Graduated with Writing Area, 100-Pack
MUHWA 4mL amber HPLC vials provide larger-volume sample containment for autosampler workflows requiring more than the standard 1.5–2mL capacity. Each vial is manufactured from Type I borosilicate glass with an amber tint that blocks UV and short-wavelength visible light, protecting light-sensitive analytes — pharmaceuticals, vitamins, natural products, photo-labile compounds — from photodegradation during extended autosampler queue times (often 12–24 hours in high-throughput labs). The 13-425 screw thread finish is the universal industry standard, compatible with autosamplers from Agilent, Waters, Shimadzu, Thermo Fisher, PerkinElmer, and CTC Analytics. Integrated graduations at 1, 2, 3, and 4mL provide instant visual volume verification, while the frosted writing patch accepts permanent marker for direct vial labeling — no need for separate labels that can peel off in solvent vapors. Each vial comes complete with a black polypropylene screw cap (pre-slit for autosampler needle access), red PTFE/white silicone septum for excellent resealing and chemical compatibility, and a 100-piece bulk pack format for economical, uninterrupted laboratory operation.
Key Features
- 4mL Amber Borosilicate Glass — Type I borosilicate glass with amber tint blocks UV (<380nm) to protect light-sensitive analytes. Ideal for vitamins (A, D, E, K), pharmaceuticals, natural products, and photo-labile compounds.
- 13-425 Standard Screw Thread — Industry-universal thread finish compatible with virtually all HPLC and UHPLC autosamplers. Interchangeable with caps and septa from major manufacturers.
- Complete with Cap and Septum — Each vial includes a pre-assembled black PP screw cap with pre-slit hole for autosampler needle, plus red PTFE/white silicone septa for excellent chemical resistance and multiple injection resealing.
- Red PTFE / White Silicone Septa — PTFE (facing sample) provides broad chemical compatibility; silicone layer provides elasticity for needle penetration and self-sealing. Rated for multiple injections per septum.
- Integrated Graduations and Writing Area — Ceramic-printed white graduations at 1, 2, 3, and 4mL. Frosted writing patch for direct permanent-marker labeling — no adhesive labels required.
- 100 pcs Bulk Economy Pack — Pre-cleaned and ready to use. Bulk format reduces per-vial cost for high-throughput labs while minimizing packaging waste.
- Wide Mouth for Easy Filling — 13-425 opening (approximately 9mm ID) accommodates standard pipette tips and syringe needles for convenient sample loading without a funnel.
Technical Specifications
| Parameter | Specification |
|---|
| Vial Volume | 4 mL (nominal) |
| Vial Material | Type I borosilicate glass (amber) |
| Cap Finish | 13-425 screw thread |
| Cap Material | Black polypropylene (PP), pre-slit |
| Septa Material | Red PTFE (sample side) / White silicone (backing) |
| Septa Thickness | ~1.5 mm (PTFE 0.25mm + silicone 1.25mm) |
| Graduations | 1, 2, 3, 4 mL (white ceramic print) |
| Writing Area | Frosted patch for permanent marker |
| Pack Size | 100 vials + 100 caps + 100 septa |
Autosampler Compatibility
| Manufacturer | Autosampler Models | Compatibility |
|---|
| Agilent | 1100/1200/1260/1290 Series, 7693A, 7683 | Yes — 13-425 tray position |
| Waters | Alliance 2695, Acquity (with adapter), 717plus | Yes — requires 4mL tray insert |
| Shimadzu | SIL-20A/30A, SIL-10/20 series | Yes — 4mL rack position |
| Thermo Fisher | UltiMate 3000, AS3000, TriPlus | Yes — 13-425 compatible |
| PerkinElmer | Clarus series | Yes |
| CTC Analytics | PAL autosampler family | Yes — 4mL tray |
PTFE/Silicone vs. Other Septa Types
| Septa Type | PTFE/Silicone (included) | PTFE-only | Silicone-only |
|---|
| Chemical Resistance | Excellent (PTFE face) | Excellent | Limited (swells in organics) |
| Resealing | Excellent (silicone backing) | Poor (plastic deformation) | Excellent |
| Multiple Injections | Yes (3–5 per septum) | No (1–2 max) | Yes (5+) |
| Pre-Slit | Yes (cap with slit) | N/A | N/A |
| Best For | General HPLC, multiple injections | Aggressive solvents, single injection | Aqueous samples only |
Applications
- Pharmaceutical Potency and Purity Testing — Analyze drug substance and drug product formulations from 4mL sample volumes. Amber glass protects light-sensitive APIs (nifedipine, riboflavin, omeprazole, vitamin D analogs) during extended autosampler queues.
- Stability Study Sample Storage — Store and analyze stability samples (ICH Q1A accelerated and long-term conditions). 4mL capacity accommodates multiple injections from a single vial for replicate analysis.
- Environmental Pollutant Analysis — PAHs, PCBs, pesticides, and herbicides dissolved in organic solvents. PTFE septa resist swelling from dichloromethane, hexane, and acetonitrile extracts.
- Food and Beverage Testing — Vitamin fortification analysis (A, D, E, K, B vitamins), preservative quantification, and mycotoxin testing. Amber glass protects light-sensitive vitamins during analysis.
- Clinical and Bioanalytical LC-MS — Therapeutic drug monitoring (TDM), pharmacokinetic (PK) studies, and metabolomics. 4mL capacity holds sufficient sample for multiple injections and re-analysis if needed.
- Natural Products Chemistry — Protect photo-labile natural products (carotenoids, flavonoids, anthocyanins, chlorophylls) during chromatographic analysis of plant extracts and marine natural products.
- High-Throughput Screening — Bulk 100-pack supports continuous operation of walk-away overnight HPLC and UHPLC systems processing hundreds of samples per batch.
Frequently Asked Questions
Q: Will these vials fit my Agilent/Waters/Shimadzu autosampler?
A: 13-425 is the universal 4mL vial standard. These vials fit virtually all autosamplers that accept 4mL vials. Verify your autosampler's vial tray configuration — some instruments require a specific 4mL vial tray insert or rack adapter (sold separately by the instrument manufacturer).
Q: What is the difference between amber and clear glass vials?
A: Amber glass contains additives that absorb UV and short-wavelength visible light (typically below 500nm), protecting light-sensitive analytes. Use amber vials for light-sensitive compounds (vitamins, some drugs, natural products). Use clear vials for all other applications where visual inspection of sample level, color, or precipitate is important.
Q: How many times can I pierce each septum?
A: The PTFE/silicone septa can typically withstand 3–5 needle piercings before the resealing performance degrades. For critical quantitative analysis, limit to 2–3 injections per septum. If septa leakage is suspected (erratic peak areas, solvent smell), replace the vial. Pre-slit caps reduce needle coring and extend septum life compared to non-slit caps that require the needle to puncture the full septum thickness.
Q: Are the vials pre-cleaned?
A: Yes, they are manufactured and packaged under clean conditions and are suitable for most HPLC applications without additional cleaning. For ultra-trace analysis (ppb/ppt levels), a solvent rinse with your mobile phase or sample solvent is recommended as good laboratory practice.
Q: Can I use these vials for GC headspace analysis?
A: No. These are standard screw-cap HPLC vials, not rated for the pressure build-up in heated headspace GC (typically 130–200°C with vapor pressures exceeding 2 bar). For headspace GC, use dedicated 20mL headspace vials with crimp caps rated for pressure. These 4mL vials are for liquid injection HPLC/LC-MS only.
