MUHWA 22×22mm Pre-Cleaned Glass Cover Slips — 2000 PCS, No. 1 Thickness
MUHWA 22×22mm glass cover slips are precision-cut, pre-cleaned square coverslips manufactured for consistent optical performance in routine microscopy, histology, and life sciences research. Each coverslip measures 22mm × 22mm × 0.13–0.17mm (No. 1 thickness), matching the design working distance of standard microscope objectives for brightfield, phase-contrast, and fluorescence imaging. The pre-cleaned surface eliminates the need for alcohol washing or lens paper polishing before use—open the box and mount directly. This bulk pack contains 10 boxes of 200 coverslips each (2000 total), providing an economical laboratory supply for high-throughput histology labs, clinical pathology departments, and university microscopy teaching courses.
Key Features
- Pre-Cleaned Manufacturing: Coverslips are cleaned during production to remove dust, oils, and manufacturing residues. Ready for immediate use in routine microscopy, H&E staining, and immunohistochemistry without additional preparation.
- No. 1 Thickness (0.13–0.17mm): The industry-standard thickness for biological microscopy. Compatible with the working distance specifications of most achromat, plan-achromat, and fluorite objectives from major microscope manufacturers including Olympus, Nikon, Zeiss, and Leica.
- Uniform Optical Quality: Manufactured from clear soda-lime glass with controlled thickness tolerance across the entire coverslip surface. Minimizes spherical aberration when used with high-NA oil immersion objectives where coverslip thickness critically affects image quality.
- Low Autofluorescence: Suitable for fluorescence microscopy applications. The glass formulation produces minimal background fluorescence in the visible spectrum (400–700 nm), compatible with DAPI, FITC, TRITC, and GFP filter sets.
- Square 22×22mm Format: The most widely used coverslip dimension in biological microscopy. Covers the central specimen area of a standard 75×25mm microscope slide while leaving room for frosted-end labeling.
- 2000 PCS Bulk Economy Pack: Ten individually boxed sleeves of 200 coverslips (2000 total). Boxed packaging protects coverslips from breakage and dust during storage. Bulk quantity reduces per-slide cost for laboratories processing hundreds of slides weekly.
Technical Specifications
| Parameter | Specification |
| Dimensions | 22mm × 22mm |
| Thickness | 0.13–0.17mm (No. 1) |
| Material | Soda-lime glass |
| Shape | Square |
| Surface | Pre-cleaned |
| Quantity | 2000 pieces (10 boxes × 200 pcs/box) |
| Autofluorescence | Low, compatible with 400–700nm fluorescence |
| Refractive Index | ~1.52 |
| Packaging | 10 individually boxed sleeves |
| Storage | Room temperature, dry environment |
Applications
- Histology & Pathology: Coverslipping H&E-stained, PAS-stained, and immunohistochemistry (IHC) tissue sections on glass slides using mounting media such as Permount, DPX, or aqueous mounting media.
- Cell Culture Microscopy: Mounting coverslips over cells cultured directly on slides or in chamber slides for brightfield and phase-contrast observation.
- Fluorescence Microscopy: Compatible with standard fluorophores (DAPI, FITC, TRITC, Texas Red, Cy3, Cy5, GFP, mCherry). Low-autofluorescence glass minimizes background signal.
- Teaching Laboratories: Bulk 2000-pack provides an economical supply for university biology, histology, and pathology practical courses where students prepare and examine dozens of slides per session.
- Materials Science: Suitable for mounting thin sections, powder samples, and microfabricated specimens for optical microscopy examination.
Frequently Asked Questions
Q: What does "No. 1 thickness" mean?
A: Coverslip thickness is classified by number: No. 0 (0.08–0.12mm), No. 1 (0.13–0.17mm), No. 1.5 (0.16–0.19mm), and No. 2 (0.17–0.25mm). No. 1 is the standard for most biological microscopy. Most microscope objectives are designed for a 0.17mm coverslip thickness. Using the wrong thickness introduces spherical aberration, particularly with high-NA dry and oil immersion objectives above NA 0.4.
Q: Are these coverslips really pre-cleaned? Do I need to wash them?
A: Yes, they are pre-cleaned during manufacturing. For routine brightfield microscopy and H&E histology, they can be used directly from the box. For high-sensitivity fluorescence microscopy (single-molecule imaging, TIRF), a quick rinse in 70% ethanol followed by air-drying is recommended as an additional precaution against dust particles that may settle during storage.
Q: Can I use these with oil immersion objectives?
A: Yes. The 0.13–0.17mm thickness matches the design specification of most oil immersion objectives (NA 1.25–1.4). Ensure the coverslip is fully sealed with mounting medium to prevent oil from seeping under the coverslip and contaminating the specimen.
Q: How are the coverslips packaged?
A: Each box contains 200 coverslips in a hinged storage box with a protective lining. The 10-box pack provides 2000 coverslips total. Individual boxes can be distributed across multiple workstations or teaching laboratory benches.
Q: What is the difference between soda-lime and borosilicate coverslips?
A: Soda-lime glass (used here) is the standard for routine microscopy—cost-effective with good optical clarity and adequate chemical durability. Borosilicate glass offers superior chemical resistance and thermal stability but at significantly higher cost. For standard histology, cell biology, and teaching applications, soda-lime coverslips provide excellent performance at an economical price point.
