MUHWA 2.2mL 96-Well Deep Well Plate — Square Wells, U-Bottom, Polypropylene, 5 Plates per Bag

MUHWA 2.2mL 96-Well Square Deep Well Plate — Maximum-Capacity U-Bottom Polypropylene Plate for High-Volume Workflows

MUHWA 2.2mL 96-well square deep well plates deliver the highest working volume (2.2 mL per well) in the standard 96-well ANSI/SLAS format, making them the ideal choice for bacterial expression cultures, protein production, large-volume sample storage, and any high-throughput protocol requiring maximum per-well capacity. The 96 square wells with U-bottom geometry facilitate complete sample recovery, efficient magnetic bead resuspension, and uniform mixing during orbital shaking. Manufactured from virgin polypropylene with excellent chemical resistance to DMSO, alcohols, and common solvents, these plates withstand autoclave sterilization, ultra-low temperature storage, and repeated freeze-thaw cycles without degradation. The 5-plate bulk bag format provides economical, high-throughput-ready supply for busy laboratories.

Key Features

• 2.2 mL Maximum Working Volume — Nearly double the capacity of standard 1.2mL deep well plates. Accommodates larger bacterial culture volumes, increased reagent volumes, and generous sample aliquots.
• 96 Square Wells, U-Bottom — Square well geometry maximizes space efficiency. U-bottom ensures complete sample aspiration with minimal dead volume and promotes uniform cell pelleting during centrifugation.
• Virgin Polypropylene (PP) — Outstanding chemical resistance to DMSO, acetonitrile, methanol, and aqueous buffers. Low extractables and leachables for LC-MS compatible sample storage.
• ANSI/SLAS Standard Format — 9 mm well pitch, 127.76 × 85.48 mm footprint. Fully compatible with automated liquid handlers, robotic systems, multichannel pipettes, and plate readers.
• Wide Temperature Range — Serviceable from -120°C (ultra-low freezer and vapor-phase LN2 storage) to +121°C (autoclave sterilization).
• 5 Plates per Bag, Non-Sterile — Economical bulk packaging. Autoclave prior to use if sterile conditions required.

Technical Specifications

Applications

• Bacterial Expression Cultures — Grow 96 parallel 1–2 mL E. coli, yeast, or insect cell cultures for recombinant protein expression screening. U-bottom promotes uniform aeration on orbital shakers.
• Large-Volume Sample Storage — Store plasma, serum, urine, CSF, and other clinical specimens in 2.2 mL aliquots. Standard plate format integrates with automated biobanking systems.
• Protein and Enzyme Preparations — Aliquot purified proteins, antibodies, and enzymes into ready-to-use single-well volumes. Reduce freeze-thaw cycles on master stocks.
• Magnetic Bead-Based Nucleic Acid Extraction — Large well volume accommodates the additional binding, washing, and elution buffer volumes required for high-yield DNA/RNA purification from large samples.
• Compound Solubility and Stability Studies — Prepare compound solutions at multiple concentrations and solvent conditions for solubility screening and forced-degradation studies.

Frequently Asked Questions

Q: Can these plates be centrifuged?
A: Yes, with appropriate plate carriers. Most microplate centrifuges accommodate ANSI/SLAS standard plates up to ~3,000 × g. Verify your centrifuge rotor specifications before exceeding 3,000 × g.

Q: Are these compatible with heat sealing?
A: Yes. Compatible with aluminum and clear heat seals, adhesive films, and reusable silicone sealing mats (96 square well format). Heat sealing provides the best evaporative loss protection for long-term storage.

Q: Will 2.2 mL fill volume cause well-to-well cross-contamination during shaking?
A: At maximum fill (2.2 mL), there is limited headspace. For vigorous orbital shaking (>300 rpm), reduce fill volume to 1.5–1.8 mL to prevent splash-over between wells. For gentle shaking or static incubation, 2.2 mL is safe.