MUHWA 1.2mL 96-Well Square Deep Well Plate — U-Bottom Polypropylene Storage Plate for High-Throughput Workflows
MUHWA 1.2mL 96-well square deep well plates provide a robust, chemically resistant platform for sample storage, compound management, nucleic acid preparation, and high-throughput screening (HTS). Each plate features 96 square wells arranged in the standard 8×12 ANSI/SLAS microplate format (9 mm pitch) with a 1.2 mL maximum working volume and U-bottom geometry for efficient sample recovery and magnetic bead-based protocols. Manufactured from virgin polypropylene (PP), the plates offer excellent chemical resistance to DMSO, acetonitrile, methanol, and common laboratory solvents — critical for compound library storage. The polypropylene construction withstands autoclave sterilization at 121°C and ultra-low temperature storage down to -120°C, making these plates suitable for both short-term benchtop use and long-term biobanking. Supplied non-sterile in bulk bags of 5 plates for economical, high-throughput laboratory operation.
Key Features
- 1.2 mL Working Volume — Generous per-well capacity accommodates sample aliquots, reaction master mixes, bacterial cultures, and compound solutions with room for mixing and pipetting.
- 96 Square Wells, U-Bottom — Square well geometry maximizes plate real estate efficiency. U-bottom (round-bottom) wells ensure complete sample aspiration and minimize dead volume — critical for precious samples and expensive reagents.
- Virgin Polypropylene (PP) — Excellent chemical resistance to DMSO (compound library storage), alcohols, acetonitrile, and aqueous buffers. Low extractables profile compatible with LC-MS and bioanalytical workflows.
- ANSI/SLAS Standard Format — 127.76 × 85.48 mm footprint with 9 mm well pitch. Compatible with automated liquid handlers (Tecan, Hamilton, Beckman, Agilent), robotic plate handlers, and multichannel pipettes.
- Ultra-Low Temperature Compatible (-120°C) — PP remains impact-resistant and dimensionally stable during freeze-thaw cycles. Suitable for long-term sample biobanking in -80°C freezers and vapor-phase LN2.
- Autoclavable (121°C, 15 psi) — Sterilize prior to use for cell culture, microbiology, and PCR workflows. Plates maintain dimensional stability after repeated autoclave cycles.
- 5 Plates per Bag, Non-Sterile — Bulk packaging minimizes per-plate cost for high-throughput labs. Non-sterile format allows user to sterilize (or not) per protocol requirements.
Technical Specifications
| Parameter | Specification |
|---|
| Well Format | 96 square wells (8×12 array) |
| Maximum Working Volume | 1.2 mL per well |
| Well Bottom Shape | U-bottom (round) |
| Material | Virgin polypropylene (PP) |
| Plate Dimensions | 127.76 × 85.48 mm (ANSI/SLAS standard) |
| Well Pitch | 9 mm |
| Temperature Range | -120°C to +121°C |
| Autoclavable | Yes (121°C, 15 psi, 20 min) |
| Sterility | Non-sterile |
| Pack Size | 5 plates per bag |
Automation Compatibility
| Equipment Type | Examples | Compatibility |
|---|
| Automated Liquid Handlers | Tecan Freedom EVO, Hamilton STAR, Beckman Biomek, Agilent Bravo | Yes — ANSI/SLAS format |
| Multichannel Pipettes | 8-channel and 12-channel (200µL, 1000µL) | Yes — 9mm pitch |
| Plate Readers | Bottom-reading and top-reading fluorescence/absorbance | Yes — clear PP (absorbance mode requires clear-bottom plates) |
| Centrifuges with Plate Rotors | Beckman, Eppendorf, Sorvall plate carriers | Yes — standard plate dimensions |
| Automated Plate Sealers | Heat sealers and adhesive film applicators | Yes |
Applications
- Compound Library Storage — Store small-molecule compound libraries dissolved in DMSO at -20°C or -80°C. PP chemical resistance prevents compound adsorption and leachable contamination.
- Nucleic Acid Extraction and Purification — Magnetic bead-based DNA/RNA extraction in 96-well format. U-bottom wells facilitate bead pelleting and resuspension with minimal dead volume.
- Sample Aliquoting and Archiving — Prepare replicate sample aliquots for distribution to collaborators, core facilities, or long-term biobank storage.
- Bacterial and Yeast Culture Growth — Grow 96 parallel cultures for protein expression screening, clone selection, or growth curve analysis. U-bottom promotes uniform aeration during orbital shaking.
- ELISA and Immunoassay Reagent Storage — Store coating antibodies, blocking buffers, and detection reagents in ready-to-use 96-well format for streamlined assay setup.
- PCR and qPCR Master Mix Preparation — Pre-dispense master mixes and template dilutions in 96-well format for high-throughput PCR setup using multichannel pipettes.
Frequently Asked Questions
Q: Are these plates sterile?
A: No, they are supplied non-sterile. Autoclave at 121°C, 15 psi for 20 minutes if sterility is required for cell culture or microbiology applications. The PP material withstands standard autoclave cycles without deformation.
Q: Can these plates be sealed?
A: Yes. Compatible with adhesive sealing films, heat seals (aluminum or clear), and reusable silicone sealing mats (96 square well format). For long-term storage, we recommend heat sealing for maximum evaporative loss prevention.
Q: What is the difference between U-bottom and V-bottom wells?
A: U-bottom (round-bottom) wells provide gentler sample handling and easier magnetic bead resuspension with less bead trapping. V-bottom wells concentrate samples into a sharper point for maximum recovery of very small volumes. U-bottom is preferred for most sample storage and bead-based protocol applications.
Q: Will DMSO or other organic solvents damage the plates?
A: Polypropylene has excellent chemical resistance to DMSO, making these plates ideal for compound library storage. They also resist methanol, ethanol, acetonitrile, and aqueous buffers. Avoid prolonged exposure to chlorinated solvents (dichloromethane, chloroform) and strong oxidizing acids which can degrade PP over time.
