MUHWA 100-Piece Glass Vial Inserts — 150µL Conical Bottom for 2mL Large-Opening Autosampler Vials, 5.0mm ID
MUHWA 100-piece glass vial inserts provide the essential solution for analyzing small sample volumes in standard 2mL large-opening autosampler vials. Each insert features a 150µL capacity with a precision conical bottom that concentrates limited samples into a minimal dead volume (<5µL residual), enabling the autosampler needle to aspirate nearly the entire sample — critical for precious, limited, or expensive analytes. The 5.0 mm inner diameter and 9 mm outer diameter ensure a secure fit inside 2mL vials with large (9mm) openings while the borosilicate glass construction delivers excellent chemical inertness for both aqueous and organic HPLC/GC mobile phases. The conical internal geometry also facilitates complete sample recovery during manual transfers and minimizes surface area for analyte adsorption compared to flat-bottom inserts. The bulk 100-piece pack provides exceptional value for high-throughput pharmaceutical, environmental, and clinical analytical laboratories running hundreds of samples daily.
Key Features
- 150µL Conical Bottom Capacity — Optimized for sample volumes from 10–150µL. The sharp conical tip pools liquid at the lowest point for maximum needle recovery with <5µL dead volume.
- 100 pcs Economy Bulk Pack — Per-insert cost is a fraction of brand-name alternatives. Ideal for high-throughput labs consuming inserts by the hundreds weekly.
- 5.0 mm ID × 9 mm OD — Standard dimensions for 2mL large-opening (9mm) screw-cap and crimp-cap autosampler vials. Fits Agilent, Waters, Shimadzu, Thermo, and most major brands.
- Borosilicate Glass (Type I, Class A) — Chemically inert to aqueous buffers, acetonitrile, methanol, and common HPLC mobile phases. Low extractables profile complies with USP <660> and EP 3.2.1 requirements.
- Minimal Analyte Adsorption — Smooth glass surface with low surface energy minimizes non-specific binding of basic compounds, peptides, and small molecules — reduces carryover between injections.
- Universal Vial Compatibility — Drop-in compatible with most 2mL large-opening vials (9mm neck). Use with or without a plastic spring or spacer at the bottom of the vial for height adjustment.
Technical Specifications
| Parameter | Specification |
|---|
| Capacity | 150 µL (conical bottom) |
| Inner Diameter (ID) | 5.0 mm |
| Outer Diameter (OD) | 9 mm (fits 9mm large-opening vials) |
| Material | Borosilicate glass (Type I, Class A) |
| Bottom Shape | Conical (sharp taper) |
| Dead Volume | <5 µL |
| Pack Size | 100 inserts |
| Sterility | Non-sterile |
Applications
- HPLC and UHPLC Analysis — Maximize injection volume from limited samples. The conical bottom ensures the autosampler needle reaches the sample pool for consistent, bubble-free aspiration.
- GC and GC-MS Sample Preparation — Concentrate volatile analytes in minimal solvent volume. Borosilicate glass withstands organic solvents (dichloromethane, hexane, ethyl acetate) without leaching.
- Pharmaceutical Impurity Testing — Analyze low-concentration impurities and degradation products from limited-volume stability samples. Minimal dead volume preserves every microliter.
- Environmental Trace Analysis — Concentrated extracts from water, soil, and air samples. Run duplicate or triplicate injections from a single small-volume extract.
- Clinical and Forensic Toxicology — Drug and metabolite analysis from small-volume blood, urine, and tissue extracts where sample is precious or irreplaceable.
- Peptide and Protein Analysis — Low-binding borosilicate glass minimizes adsorptive losses of hydrophobic peptides during LC-MS analysis.
Frequently Asked Questions
Q: Do I need a spring or spacer at the bottom of the vial?
A: Yes, in most cases. A small plastic spring, metal spring, or spacer at the bottom of the 2mL vial raises the insert to the correct height so the autosampler needle can reach the conical bottom without contacting the vial wall. Some vials have built-in spacers; check your vial specifications.
Q: What is the maximum fill volume?
A: 150µL at the conical rim. For best autosampler performance, fill to 100–140µL. Filling above 150µL may cause sample to spill into the outer vial, defeating the purpose of the insert.
Q: Are these inserts pre-cleaned?
A: These are standard-grade borosilicate glass inserts. For trace-level analysis (ppb/ppt), we recommend solvent-rinsing inserts with your mobile phase or sample solvent before first use to remove any manufacturing residues.
Q: Can I reuse these inserts?
A: They are designed and priced as disposables for single-use to eliminate cross-contamination risk. Reuse is technically possible if thoroughly cleaned between samples, but not recommended for quantitative analysis due to carryover concerns.
