MUHWA 0.5 mL Graduated Microcentrifuge Tubes — Polypropylene Snap Cap Microtubes, 1000-Piece Pack
MUHWA 0.5 mL microcentrifuge tubes are manufactured from premium virgin polypropylene (PP) in a Class 100,000 cleanroom environment, ensuring RNase-free, DNase-free, and non-pyrogenic quality suitable for sensitive molecular biology workflows. Each tube features easy-to-read raised graduation markings at 0.1 mL, 0.25 mL, and 0.5 mL for quick volume estimation, plus a frosted writing area for sample identification with permanent markers. The attached snap cap provides a secure, leak-resistant seal that withstands centrifugation forces up to 20,000 × g, while the cap's ergonomic tab design enables easy one-handed opening and closing. With 1,000 tubes per pack, this bulk format delivers exceptional value for high-throughput research, clinical, and teaching laboratories. The thin-wall construction ensures efficient heat transfer during thermal cycling (PCR) and rapid temperature equilibration during incubation.
Key Features
- RNase/DNase-Free & Non-Pyrogenic — Manufactured and packaged in a controlled cleanroom environment. Certified free of detectable RNase, DNase, human DNA, and pyrogens — safe for PCR, qPCR, RT-PCR, and enzymatic reactions.
- Raised Graduation Markings — Clear volumetric indicators at 0.1, 0.25, and 0.5 mL molded directly into the tube wall. Will not fade, rub off, or dissolve in organic solvents unlike printed markings.
- Secure Snap Cap Design — Attached polypropylene cap with a positive-lock mechanism that produces an audible click upon closure. Leak-tested to 20,000 × g to prevent sample loss during high-speed centrifugation.
- Frosted Writing Area — Large matte-finish patch on the tube body accepts permanent marker, pencil, and cryo-marker for durable sample identification. Will not smudge when handled with gloved hands or when tubes are wet.
- Thin-Wall Construction — Optimized wall thickness balances mechanical strength with efficient thermal conductivity, enabling rapid heat transfer during thermal cycling and temperature equilibration.
- Bulk 1000-Piece Pack — Economical bulk format reduces per-tube cost and packaging waste. Ideal for high-throughput labs, core facilities, and teaching laboratories with high daily tube consumption.
- Autoclavable (121°C, 15 psi, 20 min) — Polypropylene construction withstands standard autoclave sterilization cycles. Tubes remain clear and dimensionally stable after repeated autoclaving.
- Wide Temperature Range — Operational from -80°C (ultra-low freezer storage) to 121°C (autoclave). Tubes remain impact-resistant at cryogenic temperatures for frozen sample storage.
Technical Specifications
| Parameter | Specification |
|---|
| Maximum Volume | 0.5 mL (500 µL) |
| Tube Material | Virgin polypropylene (PP) |
| Cap Material | Polypropylene (attached snap cap) |
| Graduation Markings | Raised: 0.1, 0.25, 0.5 mL |
| Maximum RCF | 20,000 × g (with cap securely closed) |
| Temperature Range | -80°C to +121°C |
| Autoclavable | Yes (121°C, 15 psi, 20 minutes) |
| Sterility | Non-sterile (can be autoclaved prior to use) |
| Certifications | RNase-free, DNase-free, non-pyrogenic |
| Pack Size | 1,000 tubes (bulk bag) |
| Manufacturing Environment | Class 100,000 cleanroom |
Applications
- PCR and qPCR Sample Preparation — RNase/DNase-free quality and thin-wall construction make these tubes ideal for preparing and aliquoting PCR master mixes, template DNA, and reaction components.
- Microcentrifugation — Leak-resistant snap cap and 20,000 × g rating support routine pelleting, spin-down, phase separation, and buffer exchange protocols in standard microcentrifuges.
- Sample Storage (-20°C and -80°C) — Frosted writing area enables clear labeling for frozen sample archives. Tubes remain mechanically robust at cryogenic temperatures.
- Enzymatic Reactions — Non-pyrogenic, nuclease-free quality ensures restriction digests, ligations, phosphorylation, and other enzymatic reactions proceed without inhibition or contamination.
- Protein and Nucleic Acid Quantification — Graduation markings facilitate rapid volume checks during sample aliquoting for NanoDrop, Qubit, or spectrophotometer-based quantification workflows.
- Reagent Aliquoting — Convenient for preparing single-use aliquots of enzymes, dNTPs, primers, antibodies, and other precious reagents to minimize freeze-thaw cycles on master stocks.
- Teaching Laboratories — Bulk 1,000-tube economics make these ideal for university biology, biochemistry, and molecular biology practical courses with high student throughput.
MUHWA Microcentrifuge Tube Family
| Model | Volume | Pack Size | Best For |
|---|
| 0.5 mL Snap Cap (this product) | 0.5 mL | 1,000 pcs | PCR prep, aliquoting, small-volume storage |
| 1.5 mL Snap Cap | 1.5 mL | 500 / 1,000 pcs | General microcentrifuge work, DNA/RNA prep |
| 2.0 mL Snap Cap | 2.0 mL | 500 pcs | Larger reaction volumes, protein work |
| 0.2 mL PCR Strip Tubes | 0.2 mL | 120 strips | Direct PCR/qPCR amplification |
Frequently Asked Questions
Q: Are these tubes sterile?
A: These tubes are supplied non-sterile in bulk packaging. They are manufactured in a cleanroom and certified RNase/DNase-free, non-pyrogenic. For sterile applications, autoclave at 121°C, 15 psi for 20 minutes prior to use. The polypropylene material withstands this cycle without deformation.
Q: Can these tubes be used in a thermal cycler (PCR machine)?
A: While these 0.5 mL tubes physically fit some older thermal cycler blocks, we recommend dedicated PCR strip tubes or PCR plates for optimal thermal contact and consistent results. These tubes are better suited for PCR reagent preparation and aliquoting rather than direct amplification.
Q: What is the maximum centrifugation speed?
A: 20,000 × g with the snap cap securely closed. Always ensure the cap is fully engaged (audible click) before centrifugation. For speeds above 20,000 × g, consider screw-cap or O-ring sealed tubes designed for ultracentrifugation.
Q: Will markers or labels adhere to the frosted area at -80°C?
A: The frosted writing patch accepts alcohol-resistant lab markers and cryo-markers. Standard permanent markers (e.g., Sharpie) work at room temperature but may smudge with condensation. For -80°C storage, we recommend using cryo-specific markers or adhesive cryo-labels for maximum durability.
Q: How do these compare to Eppendorf or other brand-name tubes?
A: MUHWA microcentrifuge tubes meet equivalent quality standards (RNase/DNase-free, non-pyrogenic, 20,000 × g rating) at a significantly more economical price point, especially in the bulk 1,000-piece format. They are compatible with standard microcentrifuge rotors accepting 0.5 mL tubes.
