MUHWA 384-Well Silicone Sealing Mat — 10-Pack
MUHWA 10-pack silicone sealing mats provide a reliable, reusable sealing solution for 384 round well plates. Each mat is precision-molded from high-grade, platinum-cured silicone to form a tight, individual seal on every well. This prevents sample evaporation, airborne contamination, and well-to-well cross-contamination during incubation, shaking, cold storage, and thermal cycling.
Unlike adhesive films that leave residue and generate plastic waste, these mats can be autoclaved and reused dozens of times — reducing consumable costs and environmental impact. The 10-pack ensures your lab always has a clean mat on hand for high-throughput workflows.
Key Features
- 10 Mats per Pack: Always have a clean, sterile mat ready; economical for labs running multiple plates daily.
- Autoclavable at 121°C: Steam-sterilize between uses without material degradation; maintains sealing integrity through repeated cycles.
- Individual Well Seal: Each of the 384 wells is independently sealed, unlike flat film seals that can bridge across wells. Prevents cross-contamination and evaporative loss even with partially filled plates.
- Broad Chemical Compatibility: Inert to DMSO, methanol, acetonitrile, aqueous buffers, cell culture media, and mild acids/bases. Does not leach or swell with extended solvent exposure.
- Pierceable for Robotic Access: Compatible with automated liquid handlers; needle can pierce through the mat for sample addition, then the silicone reseals.
- Temperature Range -80°C to 121°C: Flexible and seal-tight at freezer temperatures; withstands autoclave sterilization.
- Reusable Dozens of Cycles: Unlike single-use adhesive seals, each mat lasts through multiple autoclave and use cycles.
Technical Specifications
| Material |
Platinum-cured Silicone (high grade) |
| Format |
384 round wells |
| Plate Compatibility |
Standard 384 round well plates |
| Dimensions |
12.3 × 8.3 cm |
| Sterilization |
Autoclavable at 121°C for 20 min |
| Temperature Range |
-80°C to +121°C |
| Reusability |
Dozens of autoclave cycles |
| Pierceable |
Yes (resealing silicone) |
| Sterility |
Non-sterile (autoclave before use) |
| Package |
10 mats per pack |
Applications
- Compound library storage (DMSO solutions)
- PCR and qPCR reaction setup and storage
- Enzymatic assay incubation
- Long-term sample banking at -80°C
- High-throughput screening (HTS) workflows
- Automated liquid handling and robotic sampling
- Cell-based assay incubation
Comparison: Silicone Mats vs. Adhesive Films
| Feature |
Silicone Sealing Mat |
Adhesive Film Seal |
| Reusability |
Dozens of cycles |
Single-use |
| Autoclavable |
Yes |
No |
| Residue on Plate |
None |
Adhesive residue possible |
| Pierceable |
Yes (reseals) |
Yes (does not reseal) |
| Cost per Use |
Low (reusable) |
Higher (disposable) |
| Individual Well Seal |
Per well |
Flat across all wells |
| Risk of Cross-Contamination |
Very low |
Low |
Frequently Asked Questions
Q: How many times can each mat be reused?
A: Under normal lab conditions with autoclaving between uses, each mat can be reused 20–50+ times. Replace when you notice loss of elasticity, visible cracks, or reduced sealing performance.
Q: Are these mats compatible with all 384 round well plate brands?
A: They fit standard-format 384 round well plates from most manufacturers (Corning, Nunc, Greiner, Eppendorf). Always verify fit with your specific plate model.
Q: How should I clean the mats between uses?
A: Rinse with deionized water and mild detergent, then autoclave at 121°C for 20 minutes. For organic solvent residue, soak briefly in 70% ethanol or isopropanol before autoclaving.
Q: Will DMSO or strong solvents degrade the silicone?
A: Platinum-cured silicone has excellent resistance to DMSO and most common lab solvents. Extended exposure (days to weeks) may cause minor swelling, which reverses upon drying.
Q: Can these mats be used for long-term -80°C storage?
A: Yes. The silicone remains flexible and seal-tight at -80°C. For multi-month storage, ensure the mat is properly seated and the plate is placed in a secondary container to prevent accidental dislodging.
